Regulation of Pyruvate Oxidation in Isolated Rabbit Heart Mitochondria
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چکیده
منابع مشابه
Regulation of pyruvate oxidation in isolated rabbit heart mitochondria.
Evidence is presented in support of the hypothesis that the pyruvate dehydrogenase multienzyme complex of intact rabbit heart mitochondria may be regulated by a phosphorylation-dephosphorylation mechanism. Mitochondria incubated in the presence of pyruvate plus L-malate and either ADP or uncoupler exhibit nearly identical, rapid rates of pyruvate oxidation but possess markedly different ATP lev...
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The oxidation of an optimal concentration of palmitoyl-carnitine, buffered with bovine serum albumin, by isolated rat heart mitochondria was found to give rise to an inactivation of pyruvate dehydrogenase, provided that the concentration of pyruvate present in the mitochondrial incubation was less than 250 muM. The greatest degree of inactivation was found at the lowest pyruvate concentration u...
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The morphology of rabbit heart muscle mitochondria isolated in several media has been compared by electron microscopy. The internal structure of isolated mitochondria differs from that of in situ mitochondria, with the type and degree of alteration depending on the isolation medium. Examination of the isolated mitochondria after incubation revealed that additional morphological changes occurred...
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Factors affecting regulation of the pyruvate dehydrogenase activity of isolated beef heart mitochondria were investigated. It was demonstrated that both calcium and magnesium caused a time-dependent enhancement of the activity of mitochondrial pyruvate dehydrogenase. Permeant anion addition either in presence or absence of exogenous divalent metal cations also caused an activation of this enzym...
متن کاملPyruvate oxidation system of heart muscle.
The DPNHz formed through Reaction 1 is reoxidized by another molecule of pyruvate, in the presence of lactic dehydrogenase, to yield DPN and lactate. With the catalytic amounts of CoA used there is little or no reaction unless the acetyl group is transferred from CoA to an acceptor such as orthophosphate in the presence of transacetylase, or oxalacetate in the presence of condensing enzyme. As ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44942-9